SPECIES: human, Caucasian
PATHOLOGY: trisomy 21 / Down syndrome#190685 (OMIM record)
ECACC catalog code: 87032602
Susceptibility to viruses: poliovirus 1
Description: Human Caucasian, skin, Down syndrome
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Luteinising Hormone (LH) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Finite lifespan, up to 30 passages expected.
Karyotype: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 54, IZSBS: BS CL30
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.