Version 4.0.201701 Home - Cell Index - Search - Web Sites Species - Tissues - Tumors - Pathologies - Tr. Agents Collections - Laboratories - Catalogues
For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Detroit 532 (human, Caucasian, skin, trisomy 21 / Down syndrome)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Luteinising Hormone (LH) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Finite lifespan, up to 30 passages expected.
  Passages: 9; Karyotype: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
  Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 54, IZSBS: BS CL30