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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
   P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Collection of human fibroblasts and UV sensitive CHO mutants


Two collections are available: the first contains human fibroblast strains derived from skin biopsies, the second selected mutants derived from the hamster cell line CHOK1. All cell lines have been established in the course of research programs on DNA repair mechanisms in mammalian cells. Human fibroblasts (group 1) are from patients affected with inherited defects in DNA repair processes and/or cancer proneness and from their relatives. The majority are homozygous or heterozygous for a xeroderma pigmentosum mutation (XP complementation groups A, C and D). Hamster clones (group 2) are hypersensitive to DNA damaging agents, UV light in particular; they have been selected by conventional mutagenesis, enrichment in repair defective mutants, isolation of colonies with reduced capability to synthesize DNA or to grow after UV irradiation. Both CHO mutants and human fibroblasts were characterised for survival and UDS after exposure to UV lights or to chemical mutagens, and for the frequency of chromosome aberrations. Genetic analysis of the DNA repair defects was performed by complementation studies.

Miria Stefanini
Laboratorio Genetica Umana
Istituto Genetica Biochimica ed Evoluzionistica, CNR
Via Abbiategrasso, 207
27100 Pavia Italy
Phone: +39-0382546330 Fax: +39-0382422286
E-mail: stefanini@igbe.pv.cnr.it


For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105