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TUMOR: carcinoma, cervical
ECACC catalog code: 85112701
Description: Human cervical carcinoma, HeLa characteristics
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 55
This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in depositors laboratory and is useful in transformation studies. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Natl Cancer Inst Monogr 1962;1:75
- Proc Natl Acad Sci U S A 1962;48:2026
- Science 1969;163:697 - PMID: 5762936
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.