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SPECIES: rat, NEDH
TUMOR: islet tumor
ECACC catalog code: 88031603
Description: Rat NEDH islet tumour
Viruses: See Properties
Depositor: Prof C N Hales, Department of Clinical Biochemistry, University of Cambridge, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Insulin and glucagon Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. The cells tend to grow in clumps and ribbons.
Established from NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. Can be used in place of CRI-D11 (ECACC No. 88031604).
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Endocrinol 1986;109:193 - PMID: 3011941
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.