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SPECIES: mouse, C57BL
TISSUE/ORGAN: pre B cell
DSMZ catalog code: ACC 241
Morphology: cells are very small and grow mainly in small clusters, but also singly in suspension
Description: mouse pre B cells established from the bone marrow of a CBA/C57BL mouse in 1995 confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. A. R. Mire-Sluis, National Institute for Biological Standards and Control, Potters Bar, England
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% McCoy's 5A + 15% non-heat inactivated FBS + 50 ng/ml human IL-7 + 0.4x MEM vitamins + 1x MEM non-essential amino acids + 0.5x essential amino acids + 1 mM sodium pyruvate + 0.12% sodium bicarbonate + 2 mM L-glutamine + 50 śM mercaptoethanol cells are difficult to culture! split saturated culture 1:2 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells may initially grow rather slowly for about one week; maintain at 1-3 x 106 cells/ml at 37C with 5% CO2 maximum density at ca. 3 x 106 cells/ml;
doubling time of about 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near-diploid karyotype with 11% polyploidy; 46(45- 49)<2n>XX, +5, +5, +7, -16, -20, -22, -22, +mar, del(5)(q11/12)x2, add(7)(q35)x1-2, add(10)(p11), add(14)(p11), add(15)(p11), der(19)t(9;19)(q21;q13); sdl with add(2)(q37);) * cytogenetics 2 (mar q-arm is identical to additional material on der(10)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin -, desmin -, endothel -, GFAP -, neurofilament + (20%), vimentin + (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.