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ICLC catalog code: HTL10001
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male)
DEPOSITOR: A M Bassi e S Penco, DIMES, General Pathology, Cell Cultures Lab, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; split confluent cultures 1:5 - 1:10 using trypsin/EDTA, 37C, 5% CO2, mycoplasma contamination eliminated with plasmocin; negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 91091816, DSMZ: ACC 141
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.