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ICLC catalog code: HTL07005
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: metastatic cutaneous nodule
DEPOSITOR: Dr G. Ferlazzo, Human Pathology Dept., Pad-H, University of Messina, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; split confluent cultures 1:2 - 1:4 using trypsin/EDTA, 37C, 5% CO2 Passage: 5, mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Originator of cell line: Dr P. Carrega
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.