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ICLC catalog code: HTL04002
Continuous culture, grown as suspension
single round cells and some small clusters, few cells are lightly adherent morphology
SPECIES: human, Caucasian (female, 6 month old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute megakaryoblastic
DEPOSITOR: Dr L. Pegoraro, University of Torino, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine + 10 ng/ml GM-CSF; Freezing medium: 90% FBS + 10% DMSO; Slit 1:2-1:3 every two days; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The viability may be low after thawing and need about one week to recover. The cells grow also with Iscove's MDM medium instead of RPMI and with IL-3 (10 ng/ml) instead of GM-CSF.
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: DSMZ: ACC 104
- Brit J Haematol 1988;69:359-366 - PMID: 3261598
- J Cell Physiol 1990;145:458-464 - PMID: 2273055
- Leukemia 1997;11:701-708 - PMID: 9180295
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.