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ICLC catalog code: HTL03001
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male, 58 year old)
DEPOSITOR: Dr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells could synthesize lecithin and are positive for keratin
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37°C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 86012804, IZSBS: TCL 101
- Int J Cancer 1976;17:62-70 - PMID: 175022
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.