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ICLC catalog code: HTL01019
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male, 26 year old)
TUMOR: adenocarcinoma, ductal
tumorigenic in nude mice
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA (20 min.) or scraper; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The cells are very difficult to detach and to disaggregate
Validated by: PCR analysis (confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 91112501
- Proc Natl Acad Sci USA 1990;87:4012-4016 - PMID: 90251687
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.