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SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute lymphoblastic
ECACC catalog code: 89090405
Description: Human Caucasian acute lymphoblastic leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: HLA profile studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO2; 37C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.
Karyotype: 2n = 46
A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 mths. HLA profile: A1, A2, B12, B17, Cw2.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 120, IZSBS: BS TCL12
- Cancer Res 1967;27:2479 - PMID: 4170381
- Monograph 1967;21
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.