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DSMZ catalog code: ACC 54
Morphology: epithelial-like cells, growing as adherent monolayer
Description: human kidney carcinoma established from the primary renal carcinoma of a 69-year-old man confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% McCoy's 5A + 10% FBS change medium twice every week, split confluent cultures 1:2 to 1:3 weekly using trypsin/EDTA, upon thawing grow cells immediately in 80 cm 2 ; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 cell harvest of ca. 1 x 106 cells/25 cm 2;
doubling time of ca. 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
Availability in cell line catalogues: ATCC: HTB 47, IZSBS: BS TCL8, ICLC: HTL95029
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;49:5
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.