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ECACC catalog code: 93120817
Description: Mouse neuroblastoma
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: In vitro hybridization of somatic cells
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Cultures may not form a complete monolayer before subculture is required.
CLONE NA is a subclone of Neuro-2a, a clonal line of C1300 mouse neuroblastoma cells. The cells were selected for spontaneous mutations deficient in HGPRT in the presence of 8-azaguanine.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Dev Biol 1974;39:226 - PMID: 4851982
- J Clin Microbiol 1987;25:1456 - PMID: 3305560
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.