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ECACC catalog code: 94101901
Description: Mouse hepatoma
Viruses: See Properties
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Alpha-foetoprotein and Albumin
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2x1000 - 5x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Media change three times a week.
A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice [see ref. Differentiation]. Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli and fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Biochem J 1983;214(2):309-316 - PMID: 6412698
- Differentiation 1975;4:85 - PMID: 52568
- Mol Biol Cell 1993;4(1):59-69 - PMID: 8443410
- Mutat Res 1992;278(1):11-17 - PMID: 8170368
- Somatic Cell Genet 1979;5(6):719-738 - PMID: 545721
- Toxicology letters 1994;72(1-3):53-57 - PMID: 8202956
For information, get in touch with:
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Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.