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SPECIES: human, Caucasian
TUMOR: adenocarcinoma, endometrial
ECACC catalog code: 98031101
Description: Human caucasian endometrial adenocarcinoma
Viruses: See Properties
Depositor: Dr R Hackenberg, Universitšt-Frauenklinik, Marburg, Germany, GERMANY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Androgen receptor Applications: Tumour biology studies, receptor analysis
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
MEM + 2mM glutamine + 10% FBS Split confluent cultures 1:4 i.e. seeding 1-2 x 10,000 cells / cm2 using 0.25% trypsin or trypsin / EDTA; 5% CO2; 37C
Karyotype: Near Tetraploid
MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibited by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice. This cell line has only been subject to preliminary quality control procedures. Authentication tests such as DNA fingerprinting will be performed only when this cell line is requested.
Research council deposit: No;
Price Code: C;
Availability in cell line catalogues: DSMZ: ACC 419
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.