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ICLC catalog code: ATL00001
Continuous culture, grown as monolayer
fibroblast-like and round cells lightly adherent morphology
SPECIES: rat ( newborn)
non myogenic subclone
Original line: L6 (ATCC CRL1458)
DEPOSITOR: Dr S. Adamo, Medical Istology Embriology Dept, University La Sapienza, Rome, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: L6H2 display limited adhesion, do not give rise to myotubes, grow in soft agar
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split cultures 1:10-1:20 using Trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 Passage: 7, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with MD, NP)
- Am J Physiol 1993;265:C113-C121 - PMID: 8393277
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.