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ICLC catalog code: ATL99009
Continuous culture, grown as monolayer
SPECIES: rat, Fisher 344
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: drug metabolism assays, hepatocyte studies, toxicology; low basal and prooxidant-induced lipid peroxidation. Reduced alcohol dehydrogenase (ADH) and glutathione S-transferase (GST) activities; increased aldehyde dehydrogenase (ALDH) and aldehyde reductase (ALDR) activities when 4-hydroxynonenal (4-HNE) is used as substrate
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2 x 104 cells/cm2; 37C, 5% CO2, mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with NP, PepB)
- Cancer Res 1984, 44:5219-26 - PMID: 6488182
- Cancer Res 1988, 48:7009-7012 - PMID: 3263898
- Carcinogenesis 1994, 15:1359-64 - PMID: 8033312
- Free Radic Biol Med 1999, 26:333-340 - PMID: 9895224
- In Vitro 1983, 19:191-202 - PMID: 6131863
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.