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ICLC catalog code: HTL99005
Continuous culture, grown as monolayer
SPECIES: human (male, 67 years old)
TISSUE/ORGAN: intestine, ileocecal
tumorigenic in nude mice
DEPOSITOR: Dr A. Sobrero, Udine, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production; the cell line was demonstrated by cytogenetic analysis to be identical to HRT-18 cell line
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% HS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: ECACC: 90032006, IZSBS: BS TCL 97
- Int J Cancer 1975;16:74-82
- J Natl Cancer Inst 1974;52:1101-1110
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.