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ICLC catalog code: ATL99005
Continuous culture, grown as monolayer
SPECIES: mouse, BDF/1 (female, 5 months old)
TISSUE/ORGAN: bone marrow, stromal cells
TRANSFORMED BY: murine leukemia virus
tumorigenic in syngeneic animals
DEPOSITOR: Dr A. Pessina, Cell Culture Laboratory, Institute of Microbiology, University of Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce macrophage-colony stimulating factor (M-CSF) and are highly sensitive to fibroblast growth factor (FGF)
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
McCoy's 5A + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 Passage: 197, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, Pep B)
Availability in cell line catalogues: ECACC: 94102704, DSMZ: ACC 323
- Cytotechnology 1992;8:93-102 - PMID: 1382506
- Exp Hematol 1997;25:536-541 - PMID: 9197333
- Methods Mol Cell Biol 1995;5:230-236
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.