GI-ME-N

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

GI-ME-N (human, Caucasian, bone marrow, metastatic neuroblastoma, stage IV)

ICLC catalog code: HTL98011
Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 2 years old) Karyology: chromosome marker: 1p del
TISSUE/ORGAN: bone marrow
TUMOR: metastatic neuroblastoma, stage IV
highly tumorigenic in nude mice
DEPOSITOR: Dr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: undetectable N-myc expression; the cells do not express HLA Class I

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with AST)

Bibliographic references:

Pediatr Res 1990;27:1-6 - PMID: 2296463

See homonymous cell lines in other collections and catalogues.

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105