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ICLC catalog code: ATL98006
Continuous culture, grown as suspension
semi-adherent, spherical morphology
SPECIES: mouse, AKR/J
TRANSFORMED BY: X-rays Back To Radiant Agents' WebPage
DEPOSITOR: Dr D. Iannelli, Immunology Dept, Faculty of Agriculture, Naples, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells express Lyt-2, Lyt-3 and CD4 in the cytosol, but not on the membrane; they reexpress Lyt-2, Lyt-3 and CD4 on the membrane after sorting by magnetic particles (5 cycles)
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)
- Immunogenetics 1994;40(2):154-158
- J Immunol Methods 1992;154(2):211-216
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.