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ICLC catalog code: HYL98002
Continuous culture, grown as semiadherent
round cells morphology
SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid, B cells x myeloma
DEPOSITOR: Dr G. Grassi, Experimental Institute for Industrial Cultures, Bologna, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: anti plasmacell hybridoma, obtained by fusion of spleen cells from a mouse immunized with human plasmacells with PX63Ag8-653, a mouse myeloma line; HAT resistant
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The viability of the cells can be very low after thawing; thaw with medium supplemented with 20% FBS
Validated by: isoenzymes (confirmed as mouse with MD, MPI)
- Clin Exp Immunol 1987;70:192-200 - PMID: 3319299
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.