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ICLC catalog code: ATL98012
Continuous culture, grown as suspension
SPECIES: mouse, AKR
Original line: AKR/1a murine T cell line
TRANSFORMED BY: exposed to a dose of 500 rads from an X-ray source Back To Radiant Agents' WebPage
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: useful in the study of assembly and transport of Lyt-2/3 antigen in the cell surface or to define the regions of Lyt-2/3 interacting with the corresponding antibodies and with MHC class I molecules
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; maintain cultures between 3-9x10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, MD)
Availability in cell line catalogues: ECACC: 94031011
- Immunogenetics 1994;40(2):154-158 - PMID: 7517914
- J Immunol Methods 1992;154(2):211-216 - PMID: 1401954
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.