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SPECIES: mouse, BALB/c
TUMOR: leukemia, granulocytic or myelocytic
ECACC catalog code: 92110401
Description: Mouse BALB/c granulocytic leukaemia
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Interleukin 3 (IL-3)
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-5x100,000 cells/ml; 5% CO2; 37C.
Subclone of the line Wehi 3D. Cells will not respond to G-CSF or GM-CSF to differentiate in a plaque assay.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.