VERO-B4

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

VERO-B4 (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

DSMZ catalog code: ACC 33
Morphology: adherent-elongated, fibroblast-like cells in monolayers
Susceptibility to viruses: many types of viruses
Description: monkey African green monkey kidney established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing confirmed as monkey with IEF of AST, LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, Munich, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out at ca. 0.5-1.0 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 10 x 106 cells/80 cm 2; doubling time of ca. 25 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile)

Bibliographic references:

Nippon Rinsho 1963;21:1209

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105