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SPECIES: hamster, Chinese
TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 87020406
Description: Hamster Chinese embryo lung fibroblast transformed
Viruses: See Properties
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Cell fusion studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Exp Cell Res 1978;114:69 - PMID: 658159
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.