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SPECIES: rat, Wistar-Furth
TISSUE/ORGAN: spinal cord and roots
ECACC catalog code: 85040102
Description: Rat Wistar-Furth oligodendroglioma
Viruses: See Properties
Depositor: Dr K Fields, Albert Einstein College of Medicine, Bronx, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Ethyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (ECACC No. 85081901).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Proc Natl Acad Sci U S A 1975;72:1296 - PMID: 1093176
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Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.