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ECACC catalog code: 95111735
Description: Human lymphoblast, thymidine kinase heterozygote
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,302,535
Properties: Applications: Bromodeoxyuridine resistant assays
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) or FBS. Maintain cultures between 1-9x100,000 cells/ml; 5% CO2; 37C.
The thymidine kinase heterozygote cell line TK6 has been isolated from the lymphoblastoid line HH4. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. This cell line has been shown to be negative for Bovine Viral Diarrhoea Virus (BVDV).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 8015
- Biochem Biophys Res Commun 1978;84:411 - PMID: 718692
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.