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SPECIES: mouse / mouse
DSMZ catalog code: ACC 88
Morphology: single, round cells in suspension
Description: mouse hybridoma (anti-C3d receptor and anti-EBV receptor) established by fusion of the P3X63AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with human B cell line SB; cells were reported to produce a monoclonal antibody (IgG 2a kappa) reacting with the human C3d receptor (CD21) and an EBV receptor of 145 kDa confirmed as mouse with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: obtained from ATCC (HB 135), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 15% FBS optimal density at 0.5 x 106 cells/ml, minimal density at 0.1 x 106 cells/ml; split 1:3 to 1:5; after thawing it is advised to use feeder cells for the first 7-10 days at 37C with 5% CO2 maximal density at 1.0 x 106 cells/ml;
doubling time of about 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as mouse with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
- Proc Natl Acad Sci U S A 1984;81:881-885 - PMID: 6230668
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.