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DSMZ catalog code: ACC 263
Morphology: round and elongated cells with spindle-shape extensions; the round cells contain almost no cytoplasm; cells grow adherent as monolayer
Description: human rhabdomyosarcoma in the original publication and in the ATCC catalogue this cell line is described as 'medulloblastoma'; recent studies (Stratton et al., Nature 337: 311-312, 1989; Chen et al., Nature 340: 106, 1989) and the characterization carried out at the DSMZ indicated that TE-671 are muscle cells, most likely identical to the rhabdomyosarcoma cell line RD (also known as TE32 or 130T). confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% Dulbecco's MEM + 15-20% FBS split before cells are entirely confluent, about every 5-10 days, using trypsin/EDTA; seed out about 1 x 106 cells/80 cm 2 at 37C with 5-10% CO2 cell harvest of about 4 x 106 cells/175 cm 2;
doubling time of 70-80 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5-1.0 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 ("bimodal hyper-diploid/tetraploid karyotype with 9.7% polyploidy; 43-50/86-98<4n>XX, -X, -X, +1, -2, -4, +7, +8, -9, -9, -13, - 14, -14, -15, -15, -16, -16, -17, -18, -18, -19, -19, -20, -20, -21, -21, +8-11mar, del(1)(p31), add(1)) * cytogenetics 2 ((p31), i(2p),add(3)(p11),add(5)(q35)x2, del(6)(q22)x2,add(9)(q34)x2, add(12)(q24)x2, der(13)t(6;13)(p12.1;p13)x2, add(14)(p11), add(?14)(q24), i(17q), der(19)t(17;19)(q12;p/q13)x2; ploidy resembles RD embryonal rhabdomyosarcoma) * cytogenetics 3 (TE-671 is a subline) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin-, desmin+, endothel-, GFAP-, neurofilament-, vimentin+) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- Carcinogenesis 1989;10:899 - PMID: 2650908
- Int J Cancer 1977;20:206-212 - PMID: 892931
- Nature 1989;337:311 - PMID: 2911385
- Nature 1989;340:106 - PMID: 2739733
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.