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SPECIES: mouse, C3H/Mai
ECACC catalog code: 90050802
TRANSFORMED BY: SV40
Description: Mouse C3H/Mai kidney, SV40 transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies: SV40
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:8 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 40
A culture of kidney cells from C3H/Mai mice transformed with SV40. The cells possess SV40 T-Ag. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 139
- Proc Soc Exp Biol Med 1963;114:721
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.