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DSMZ catalog code: ACC 209
Morphology: epithelial-like cells growing as monolayer and in cell clusters (when cultured at high density); cells do not grow to complete confluency
Description: human neuroblastoma clonal subline of the neuroepithelioma cell line SK-N-SH that had been established in 1970 from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Heikkilae, University of Turku, Turku, Finland
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% Dulbecco's MEM + 15-20% FBS split culture 1-2 times a week using trypsin/EDTA; seed out with 2 x 106 cells/80 cm 2 at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2;
doubling time of >55 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human near-diploid karyotype with 1.8% polyploidy; 46/47(42-48)<2n>X/XX, +7, ins(1) (q32q11q43), add(9)(q34), der(22)t(?17;22)(q22;q13)) * isoenzymes (confirmed as human with IEF of AST, MDH)
Availability in cell line catalogues: ECACC: 94030304, ICLC: HTL95013
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- Cancer Res 1990;50:3422-3428 - PMID: 2334938
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.