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TISSUE/ORGAN: adrenal cortex
ECACC catalog code: 91021201
Description: Bovine adrenal cortex, normal
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 with Fibroblast Growth Factor (FGF) (crude, 50ng/ml or pure, 500 pg/ml) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from the fasciculata-reticularis zone of the adrenal cortex taken from three normal adult cows. FGF stimulates cell division. Cells are responsive to ACTH and other steroidogenic agents. Finite lifespan; 60-70 population doublings expected. Studies have indicated that this cell line produces both detectable BVDV antigens and infectious BVDV.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1796
- Endocrinology 1982;111:919 - PMID: 6213403
- In Vitro 1987;23:247
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.