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SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
ECACC catalog code: 85112106
Susceptibility to viruses: poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human Caucasian peripheral blood lymphocyte
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin M (IgM) (Lambda light chain)
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3-4x100,000 cells/ml; 5% CO2; 37C. Maximum cell density 1-2x1,000,000 cells/ml.
Karyotype: 2n = 46, modal No 45
Derived from the peripheral blood of an apparently normal 33 year old Caucasian male. The cells secrete IgM (lambda light chain). HLA = A2, Aw33, B7, B14. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 156, IZSBS: BS CL75
- J Natl Cancer Inst 1969;43:1119 - PMID: 5353241
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.