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SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
ECACC catalog code: 95011817
Description: Human Caucasian neuroblastoma
Viruses: See Properties
Depositor: Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, NY, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chemotherapy development, chromosome studies, drug resistance, differentiation studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham's F12 (1:1) + 2mM Glutamine + 15% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 44
BE(2)-C is a clonal sub-line of SK-N-BE(2) (ECCAC No. 95011815) which was isolated from bone marrow of a 22-month-old male with disseminated neuroblastoma in 1972. They are reported to be multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine. The cells show a small, refractile morphology with short, neurite-like cell processes and tend to grow in aggregates.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1978;38:3751-3757 - PMID: 29704
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.