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TISSUE/ORGAN: ascitic fluid
TUMOR: leukemia, monocytic-macrophage
ECACC catalog code: 85062803
Description: Mouse leukaemic monocyte-macrophage
Viruses: See Properties
Depositor: Dr W Scheirer, Sandoz Forschungsinstitut GmbH, Vienna, AUSTRIA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Immunoglobulin and complement Products: Lysozyme
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37C. Remove the cells mechanically.
Established from ascites of a tumour induced in male mouse by intraperitoneal injection of Abselon Leukaemia virus (A-MuLV) cells with pinocytose neutral red and phagocytose zymosan. Cells capable of antibody-dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS (0.5ng/ml).
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 71
- Cell 1978;15:261-267 - PMID: 212198
- J Immunol 1977;119:950-954 - PMID: 894031
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.