R9

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

R9 (rat, Lewis, fibroblast, embryonic)

SPECIES: rat, Lewis
TISSUE/ORGAN: fibroblast, embryonic

ECACC catalog code: 93120214
Morphology: Fibroblast
TRANSFORMED BY: RSV-33
Description: Rat embryonic fibroblasts RSV-33 transformed
Viruses: See Properties
Depositor: Dr M Grofova/Dr S Blahova, Cancer Research Institute, Bratislava, SLOVAKIA (Originator)
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: +8; Karyotype: Near diploid
The R74 line was derived by co-cultivation of 18 day-old Lewis rat embryonic fibroblasts with cells of a chicken Rous sarcoma virus-33 (RSV-33). The R9 cell population is clearly distinguishable from RT1 and R74 cell lines because it contains flat polygonal and triangular refractile cells with a tendency to round up. It is also tumourigenic in chickens and rats.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Bibliographic references:

Folia Biol (Praha) 1992;38:90

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105