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ECACC catalog code: 95012614
TRANSFORMED BY: SV40
Description: Human post pubertal prostate normal, immortalized with SV40
Viruses: See Properties
Depositor: Dr P Berthon, Dr O Cussenot, H˘pital SAINT-LOUIS, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Dihydrotestosterone receptor Applications: Study of proliferation, oncogene activation and differentiation of adult prostatic cells and initial steps leading to transformation.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 6 -10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37C.
Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1995;62:724 - PMID: 7558421
- Int J Oncol 1995;6
- J Urol 1991;143:881
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.