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ECACC catalog code: 90111909
Susceptibility to viruses: herpes simplex * reovirus 3
Description: Racoon uterus
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: feline and canine viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO2; 37C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.
Karyotype: 2n = 38
Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 74
- Proc Soc Exp Biol Med 1958;98:574
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.