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ICLC catalog code: ATL96012
Continuous culture, grown as suspension
SPECIES: mouse, BALB/c (female)
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: fusion partner; HGPRT- and HAT sensitive; does not synthesize gamma1 chains, produces kappa light chains which are not secreted
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:10; maintain cultures between 3 and 9x105 cells/ml; rapid cell death occurs at >1x106 cells/ml. 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, G6PD)
Availability in cell line catalogues: ECACC: 94120802
- Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.