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SPECIES: human, Caucasian
ECACC catalog code: 96070808
Description: Human Caucasian oesophageal carcinoma
Viruses: See Properties
Depositor: Drs J C Rockett/A Morriss, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital, UK (Originator)
Restricted. Patent: None Specified By Depositor
Properties: Receptors: MHC class I, ICAM-1 Applications: Study of oesophageal cancer
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett's metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Br J Cancer 1997;75:258 - PMID: 9010035
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IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.