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TISSUE/ORGAN: thyroid, follicular
ECACC catalog code: 90011610
Transfected by: Transfection Back To Transfection WebPage
Description: Human thyroid follicular mutant epithelial
Viruses: See Properties
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transfection, mutation and virus studies: SV40
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33C.
Similar to Nthy-ori 3-1 (90011609) but was transfected with a temperature sensitive mutant encoding SV40 large T antigen. The cells grow at 33C.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Mol Cell Biol 1990;10:5365 - PMID: 1697930
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.