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SPECIES: mouse / rat
TUMOR: neuroblastoma x glioma hybrid
ECACC catalog code: 88112302
Description: Mouse neuroblastoma x Rat glioma hybrid
Viruses: See Properties
Depositor: Dr Nirenberg, Laboratory of Biochemical Genetics, National Institute of Health, Bethesda, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Hybridization, fusion partner and virus studies: Sendai virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 seeding at 2x100,000 cells/cm2 . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO2; 37C. The cells do produce large amounts of CO2, media changes may be necessary.
Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
- ASPET 1986;31:159
- Nature 1988;336:185 - PMID: 3185736
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.