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SPECIES: mouse, NFS/N
ECACC catalog code: 88041905
Description: Mouse Pro-B lymphoblast
Viruses: See Properties
Depositor: Mr T Littlewood, Ludwig Institute for Cancer Research, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation and virus studies: MuLV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50ÁM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Established from a lymphoma produced in an NFS/N mouse by injecting CaS-NS-7 ecotropic MuLV. Cells show pre-B cell markers and appear to be at a very early stage of B-cell differentiation.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1694
- J Immunol 1984;133:744-753 - PMID: 6330201
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.