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SPECIES: mouse, C3H/HeN
ECACC catalog code: 90102521
Description: Mouse C3H/HeN connective tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS). Split confluent cultures 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 removal by mechanical by scraping; 5% CO2; 37C.
Karyotype: 2n = 40
Derived from NCTC strain 2049 of the parent NCTC clone 1328. The parent line was taken from subcutaneous areolar and adipose connective tissue from a normal 82 day old C3H/HeN mouse.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 12
- Exp Cell Res 1962;28:381
- J Natl Cancer Inst 1959;23:1035
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.