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SPECIES: human, Caucasian
TUMOR: multiple myeloma
DSMZ catalog code: ACC 163
Morphology: single, round to oval cells or clustered in small clumps in suspension
Description: human multiple myeloma established from the pleural effusion of a 62-year-old white woman with myeloma (IgAkappa) at relapse; cells were described as follows: cells synthesize high amounts of immunoglobulin, c-myc proto-oncogene is rearranged and c-myc RNA is expressed, cells are EBV-negative; patented cell line confirmed as human with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (CRL 9068), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% RPMI 1640 + 10-20% FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 50 śM mercaptoethanol cells are very difficult to culture; maintain at a minimum of 1.0 x 106 cells/ml; after thawing (7-10 days no growth) cells grow very slowly and viability may be initially low; culture in 24-well or 6-well plates is recommended; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at 1.5 x 106 cells/ml;
doubling time of ca. 70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid with 16% polyploidy; 45(43-46)<2n>X, -X, +8, -10, -13, +mar, dup(1)(q11q25), del(1)(p11p25), del(6)(q25), der(8)t(8;?)(q23;?)x2, del(9)(p13), del(12(p12)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13- CD19- CD37- HLA-DR- cyIgG- cyIgM- cy- cy+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CRL 9068, ECACC: 95050415
- Blood 1986;67:1542-1549 - PMID: 2423157
- ibid 1988;72:1163
- Mol Cell Biol 1988;8:124 - PMID: 3275865
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.