MUTZ-1

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

MUTZ-1 (human, peripheral blood, myelodysplastic syndrome)

SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: myelodysplastic syndrome

DSMZ catalog code: ACC 13
Morphology: mononuclear cells growing as single cells or in clumps in suspension
Description: human myelodysplastic syndrome established from the peripheral blood of a 5-year-old (Turkish) girl diagnosed with myelodysplastic syndrome (refractory anemia with excess of blasts) and Fanconi anemia in 1994 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Steube, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split every 2-3 days at 1:3 to 1:8; maintain at 0.2-0.6 x 106 cells/ml; maximal density at 1.5-2.0 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 cell harvest of about 1.0-1.5 x 106 cells/ml; doubling time of about 48-55 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human near-triploid karyotype with 12% polyploidy; 68(68- 73)<3n>X, der(X), -X, add(1)(p11), der(2)t(2;?11) (q22;q23), der(3)t(3;5)(q27;q14)t(5;22)(q35;q12)x2, del(3)(p11), del(3)(q11), del(5)(q13/q33)x1-2, der(6)t(3;6)(p23;) * cytogenetics 2 (der(7)t(7;11)(q35;q13), der(8)t(1;8)(q11;q24), add(9)(q34), del(9)(p13), der(14)t(14;?)(q23;?), der(14)t(14;?)(p11;?)t(1;?)(q11;?), add(14)(p11), der(15)t(5;15)(p13;p11)x2, der(16)t(7;16)(q21.1;q24), add(18)(p11), add(21)(p11); o l) * cytogenetics 3 (second cell line reported with MDS and 5q-; 1q11 rearrangements typical of transforming Fanconi anemia) * immunological analysis (CD3- CD7+ CD10+ CD13- CD19+ CD20- CD37+ HLA-DR+ cy/smIgG- cyIgM+ smIgM- cy/smkappa- cy/smlambda-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105