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TISSUE/ORGAN: bone marrow
TUMOR: leukemia, T cell
DSMZ catalog code: ACC 105
Morphology: spherical cells growing as clumps in suspension
Description: human T cell leukemia established from the bone marrow of a 2-year-old boy at diagnosis of acute lymphoblastic leukemia (ALL) prior to therapy in 1972; cells were reported as carrying the MLL-AFX and AFX-MLL fusion genes; retrospectively, the original diagnosis was T-ALL confirmed as human with IEF of AST, LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. A. Karpas, MRC Centre, Cambridge, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split saturated cultures 1:2 to 1:3 every 2-3 days; seed out at ca. 0.4-0.5 x 106 cells/ml; optimal cell density at ca. 0.5-2.0 x 10 6 cells/ml at 37C with 5% CO2 maximal cell density at ca. 2.5 x 106 cells/ml;
doubling time of ca. 50-100 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near tetraploid with hyperdiploid stem line; 47(45-50)<2n>XX, +m, del(9)(p11-12), t(9;?)(p11;?), t(12;?)(q21;?),t(13;?)(q14;?); karyotype does not correspond to that of original description) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19- CD37+ HLA-DR+ cy/sIgM- cyIgG-sIgG+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
- Blood 1982;60:1305-1308 - PMID: 6982733
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.