MiCL1 (S+L-)

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

MiCL1 (S+L-) (mustela vison (mink), lung)

SPECIES: mustela vison (mink)
TISSUE/ORGAN: lung

ECACC catalog code: 86020603
Morphology: Fibroblast-like
TRANSFORMED BY: Moloney-MSV
Endogenous viruses: contains Moloney-MSV
Susceptibility to viruses: CCC feline primate virus * M7 feline primate virus * RD114 * type C * type C, feline leukemia * xenotropic murine leukemia
Description: Mink lung, Moloney murine sarcoma virus infected
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 17; Karyotype: 2n = 30
A clone of the mink lung cell line Mv.1.Lu. Isolated leukaemia from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 64.1, ICLC: ATL97001

Bibliographic references:

Science 1976;192:1143 - PMID: 179144
Virology 1975;67:288 - PMID: 170737
Virology 1975;67:344 - PMID: 52940

See homonymous cell lines in other collections and catalogues.

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105