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SPECIES: human, Caucasian
ECACC catalog code: 85062806
Description: Human Caucasian pancreatic carcinoma
Viruses: See Properties
Depositor: Dr W Scheirer, Sandoz Forschungsinstitut GmbH, Vienna, AUSTRIA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and enzymatic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No. 61; hypotriploid
MIA-Pa-Ca-2 is an established cell line from an undifferentiated human pancreatic carcinoma. The tumour was taken from a 65 year old Caucasian male. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy, have a tendency to grow on top of other cells, eventually growing free in suspension. Sensitive to L-Asparaginase.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1420
- Int J Cancer 1977;19:128-135
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.